The Nikon Small World
Photomicrography Competition lets us see beyond the capabilities of our
unaided eyes. Almost 2000 entries from 70 countries vied for
recognition in the 37th annual contest, which celebrates photography
through a microscope. Images two through 21 showcase the contest's
winners in order, and are followed by a selection of other outstanding
works. Scientists and photographers turned their attention on a wide
range of subjects, both living and man-made, from lacewing larva to
charged couple devices, sometimes magnifying them over 2000 times their
original size. -- Lane Turner
Wim
van Egmond of the Micropolitan Museum in Rotterdam, Netherlands
photographed a Leptodora kindtii (giant waterflea) eye from a living
specimen using the differential interference contrast method. (Wim van
Egmond)
Dr.
Igor Siwanowicz of the Max Planck Institute of Neurobiology in
Martinsried, Germany shot "Portrait of a Chrysopa sp. (green lacewing)
larva" at 20x magnification using the confocal method. (Dr. Igor
Siwanowicz)
A
blade of grass at 200x magnification by Dr. Donna Stolz of the
University of Pittsburgh using confocal stack reconstruction and
autofluorescence. (Dr. Donna Stolz)
A
living specimen of Melosira moniliformis at 320x magnification imaged
with differential interference contrast by Frank Fox of Fachhochschule
Trier in Trier, Rheinland-Pfalz, Germany. (Frank Fox)
Intrinsic
fluorescence in Lepidozia reptans (liverwort) at 20x magnification in a
live mount with confocal microscopy by Dr. Robin Young of the
University of British Columbia in Vancouver. (Dr. Robin Young)
The
surface of a microchip in a 3D reconstruction at 500x magnification
using incident light and Normarski interference contrast by Alfred
Pasieka of Germany. (Alfred Pasieka)
Cracked
gallium arsenide solar cell films at 50x magnification using the
brightfield method by Dennis Callahan of the California Institute of
Technology in Pasadena. (Dennis Callahan)
A
mouse nerve fiber layer on a retinal flatmount at 40x magnification by
Gabriel Luna of the UC Santa Barbara Neuroscience Research Institute in
Santa Barbara, Calif. using the laser confocal scanning method.
(Gabriel Luna)
Graphite-bearing
granulite from Kerala, India in polarized light at 2.5x magnification
by Dr. Bernardo Cesare of the Department of Geosciences in Padova,
Italy. (Dr. Bernardo Cesare)
A
marine copepod, Temora longicornis, at 10x magnification imaged by Dr.
Jan Michels of Christian-Albrechts-Universität zu Kiel in Kiel, Germany
using the confocal process with autofluorescence and Congo red
fluorescence. (Dr. Jan Michels)
Joan
Rohl of the Institute for Biochemistry and Biology in Potsdam, Germany
used differential interference contrast to capture a freshwater water
flea, Daphnia magna, at 100x magnification. (Joan Rohl)
An
ant head magnified ten times and photographed by Dr. Jan Michels of
Christian-Albrechts-Universität zu Kiel in Kiel, Germany using the
confocal method with autofluorescence. (Dr. Jan Michels)
Thomas
Deerinck of the National Center for Microscopy and Imaging Research in
La Jolla, Calif. captured HeLa (cancer) cells at 300x magnification with
2-photon fluorescence. (Thomas Deerinck)
A
curare vine (Chondrodendron tomentosum) in cross-section at 45x
magnification was shot by Dr. Stephen S. Nagy of Montana Diatoms in
Helena, Montana using the brightfield method and digitally inverted.
(Dr. Stephen S. Nagy)
Using reflected light, Yanping Wang of the Beijing Planetarium in Beijing captured sand at 4x magnification. (Yanping Wang)
Lobe
coral (Porites lobata) displays tissue pigmentation response with red
fluorescence and epifluorescence with triple band (U/B/G) excitation in
this image by James H. Nicholson of the Coral Culture and Collaborative
Research Facility, NOAA/NOS/NCCOS/CCEHBR & HML in Charleston, S.C.
(James H. Nicholson)
Dr.
Christopher Guerin of the Flanders Institute of Biotechnology in Ghent,
Belgium photographed cultured cells growing on a bio-polymer scaffold
using the confocal method at 63x magnification. (Dr. Christopher
Guerin)
Dr.
Witold Kilarski of the EPFL-Laboratory of Lymphatic and Cancer
Bioengineering in Lausanne, Switzerland shot Litomosoides sigmodontis
(filaria worms) inside lymphatic vessels of the mouse ear at 150x
magnification using aFluorescent confocal microscopy. (Dr. Witold
Kilarski)
The
venation network of a young quaking aspen leaf (Populus tremuloides)
was magnified 4 times by Benjamin Blonder and David Elliott of the
University of Arizona in Tucson utilizing a brightfield image of
safranin-stained tissue. (Benjamin Blonder and David Elliott)
Dr.
Donna Stolz of the University of Pittsburgh assembled a wreath collage
of mammalian cells stained for various proteins and organelles magnified
from 220x to 2000x. (Dr. Donna Stolz)
Douglas
Moore of the University of Wisconsin - Stevens Point used
stereomicroscopy and fiber optics to photograph unpolished agatized ca.
150 million years old dinosaur bone cells magnified 42 times. (Douglas
Moore)
A
freshwater shrimp eye and head shot with image stacking photography by
Jose R. Almodovar at the Microscopy Center, Biology Department, UPR
Mayaguez Campus in Mayaquez, Puerto Rico. (Jose R. Almodovar)
Bladderwort
bladder (Utricularia gibber) photographed with the darkfield method and
magnified 40x by Jose R. Almodovar at the Microscopy Center, Biology
Department, UPR Mayaguez Campus in Mayaquez, Puerto Rico. (Jose R.
Almodovar)
A
confocal image of giant liposomes of pulmonary surfactant was magnified
40 times by Dr. Jorge Bernardino de la Serna of MEMPHYS - Center for
Biomembrane Physics, Department of Biochemistry and Molecular Biology in
Odense, Denmark. (Dr. Jorge Bernardino de la Serna)
A
confocal image of a reconstruction of a fruit fly (Drosophila sp.)
nervous system by Dr. Jana Boerner of Florida Atlantic University in
Boca Raton, Fla. (Dr. Jana Boerner)
Using
laser-triggered high-speed macrophotography, Dr. John H. Brackenbury of
the University of Cambridge in Cambridge, UK captured a water droplet
containing a pair of mosquito larvae. (Dr. John H. Brackenbury)
Jonathan
Franks of the University of Pittsburgh used the confocal method with
autofluorescence to capture algae biofilm. (Jonathan Franks)
The
embryonic pectoral fin of a whitespotted bamboo shark (Chiloscyllium
plagiosum) was photographed by Dr. Andrew Gillis of the University of
Cambridge using stereomicroscopy with fiber optic lighting. (Dr. Andrew
Gillis)
Dr.
Marta Guervos of the Image Processing Unit, Scientific-Technical
Facilities, University of Oviedo in Asturias, Spain photographed Acacia
dealbata (Silver wattle tree) anther using the confocal method with
autofluorescence. (Dr. Marta Guervos)
Confocal
image of Clausidium sp. nov., female with egg sacs, ventral view was
made by Dr. Terue Kihara of the German Centre for Marine Biodiversity
Research (DZMB) in Senckenberg am Meer, Germany. (Dr. Terue Kihara)
An
antique mount of a blowfly (Calliphoridae) proboscis was photographed
by Dr. Davis Linstead of Kent, UK with differential interference
contrast. (Dr. David Linstead)
The
double compound eyes of a male St. Mark's fly (Bibio marci)
photographed with reflected (episcopic) diffuse illumination by Dr.
David Maitland of Feltwell, UK. (Dr. David Maitland)
The
egg of a red admiral butterfly (Vanessa atalanta) in stinging nettle
(Urtica dioica) trichomes photographed by David Millard of Austin, Texas
with diffuse incident illumination. (David Millard)
Marek Mis of Suwalki, Poland used polarized light to photograph green algae (Spirogyra sp.) filaments. (Marek Mis)
The
eyes (anterior lateral and median) of a jumping spider photographed in
reflected light by Walter Piorkowski of South Beloit, Illinois. (Walter
Piorkowski)
A
confocal image of a charge coupled device (CCD) sensor, direct surface
view, magnified 1000 times by Kevin Smith of MetPrep Ltd. in
Warwickshire, UK. (Kevin Smith)
A
water flea (Daphnia sp.) and green algae (Volvox sp.) captured with
darkfield and flash by Dr. Ralf Wagner of Dusseldorf, Germany. (Dr.
Ralf Wagner)
The
mouth of a common fly photographed with fiber optic illumination by Dr.
Havi Sarfaty of the Israeli Veterinary Association. (Dr. Havi Sarfaty)
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